Recombinant humaan Cathepsine L-eiwit

CTSL, also known as cathepsin L1 isoform 1, is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. Multiple alternatively spliced transcript variants have been found for this gene. Recombinant Human CTSL protein was expressed in E. coli and purified by using conventional chromatography techniques.

Procathepsin L, Cathepsin L1, Major excreted protein, MEP, CTSL, CTSL1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

38.3 kDa (339aa) confirmed by MALDI-TOF

Cathepsin L, Cathepsin L1 isofrorm 1, CATL, CTSL1, MEP, ATGP3536-10 µg, ATGP3536-20 µg, ATGP3536-50 µg, ATGP3536-100 µg, ATGP3536-250 µg, ATGP3536-500 µg, ATGP3536-1 mg, ATGP3536-10, ATGP3536-20, ATGP3536-50, ATGP3536-100, ATGP3536-250, ATGP3536-500, ATGP3536-1

Bauer Y., et al. (2011) Hypertension. 57 (4) :795-801; ; Wei DH., et al. (2013) Int J Mol Med. 31 (2) :400-6

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Neuroscience

NP_001244901

P07711

Human

MGSSHHHHHH SSGLVPRGSH MGSTLTFDHS LEAQWTKWKA MHNRLYGMNE EGWRRAVWEK NMKMIELHNQ EYREGKHSFT MAMNAFGDMT SEEFRQVMNG FQNRKPRKGK VFQEPLFYEA PRSVDWREKG YVTPVKNQGQ CGSCWAFSAT GALEGQMFRK TGRLISLSEQ NLVDCSGPQG NEGCNGGLMD YAFQYVQDNG GLDSEESYPY EATEESCKYN PKYSVANDTG FVDIPKQEKA LMKAVATVGP ISVAIDAGHE SFLFYKEGIY FEPDCSSEDM DHGVLVVGYG FESTESDNNK YWLVKNSWGE EWGMGGYVKM AKDRRNHCGI ASAASYPTV