Recombinant humaan CBR4-eiwit

CBR4 belongs to the short-chain dehydrogenase/reductase family. The formation of heteroteramer with HSD17B8 has NADH-dependent 3-ketoacyl-acyl carrier protein reductase activity for o- and p-quinones. It plays a role in biosynthesis of fatty acids in mitochondria and has broad substrate specificity and reduces 9, 10-phenanthrenequinone, 1, 4-benzoquinone and various other o-quinones and p-quinones (in vitro) . Recombinant human CBR4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Carbonyl reductase family member 4, Carbonyl reductase family member 4, Quinone reductase CBR4, 3-oxoacyl-[acyl-carrier-protein] reductase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

27.5 kDa (257aa) confirmed by MALDI-TOF

CBR4, Carbonyl reductase family member 4, Carbonyl reductase family member 4, Quinone reductase CBR4, 3-oxoacyl-[acyl-carrier-protein] reductase, ATGP0781-10 µg, ATGP0781-20 µg, ATGP0781-50 µg, ATGP0781-100 µg, ATGP0781-250 µg, ATGP0781-500 µg, ATGP0781-1 mg, ATGP0781-10, ATGP0781-20, ATGP0781-50, ATGP0781-100, ATGP0781-250, ATGP0781-500, ATGP0781-1

Endo S., et al. (2008) Biochem Biophys Res Commun. 26; ;377 (4) :1326-30; ; Chen Z., et al. (2009) FASEB J. 23:3682-3691;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 5mM DTT, 200 mM NaCl

Redox Proteins

AAH21973.1

Q8N4T8

Human

MDKVCAVFGG SRGIGRAVAQ LMARKGYRLA VIARNLEGAK AAAGDLGGDH LAFSCDVAKE HDVQNTFEEM EKHLGRVNFL VNAAGINRDG LLVRTKTEDM VSQLHTNLLG SMLTCKAAMR TMIQQQGGSI VNVGSIVGLK GNSGQSVYSA SKGGLVGFSR ALAKEVARKK IRVNVVAPGF VHTDMTKDLK EEHLKKNIPL GRFGETIEVA HAVVFLLESP YITGHVLVVD GGLQLIL