Recombinant humaan CBX1-eiwit

CBX1 is a member of the heterochromatin protein family and enriched in the heterochromatin and associated with centromeres. It is recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Interaction with lamin B receptor (LBR) can contribute to the association of the heterochromatin with the inner nuclear membrane. This protein may play an important role in the epigenetic control of chromatin structure and gene expression. Recombinant human CBX1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Chromobox protein homolog 1, CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, M31, MOD1, p25beta

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

23.6 kDa (205aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CBX1, Chromobox protein homolog 1, CBX, HP1-BETA, HP1Hs-beta, HP1Hsbeta, M31, MOD1, p25beta, ATGP1113-10 µg, ATGP1113-20 µg, ATGP1113-50 µg, ATGP1113-100 µg, ATGP1113-250 µg, ATGP1113-500 µg, ATGP1113-1 mg, ATGP1113-10, ATGP1113-20, ATGP1113-50, ATGP1113-100, ATGP1113-250, ATGP1113-500, ATGP1113-1

Furuta K., et al. (1997) Chromosoma. 106 (1) :11-9.; ; Minc E., et al. (1999) Chromosoma. 108 (4) :220-34.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 2mM DTT, 0.1M NaCl.

Other

NP_006798

P83916

Human

MGSSHHHHHH SSGLVPRGSH MGKKQNKKKV EEVLEEEEEE YVVEKVLDRR VVKGKVEYLL KWKGFSDEDN TWEPEENLDC PDLIAEFLQS QKTAHETDKS EGGKRKADSD SEDKGEESKP KKKKEESEKP RGFARGLEPE RIIGATDSSG ELMFLMKWKN SDEADLVPAK EANVKCPQVV ISFYEERLTW HSYPSEDDDK KDDKN