Recombinant humaan CBX3-eiwit

CBX3, also known as chromobox protein homolog 3, seems to be involved in transcriptional silencing in heterochromatin-like complexes. This protein recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. It involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Recombinant human CBX3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Chromobox protein homolog 3, HECH, HP1-GAMMA, HP1Hs-gamma

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.4 kDa (207aa) confirmed by MALDI-TOF

CBX3, ATGP1456-10 µg, ATGP1456-20 µg, ATGP1456-50 µg, ATGP1456-100 µg, ATGP1456-250 µg, ATGP1456-500 µg, ATGP1456-1 mg, ATGP1456-10, ATGP1456-20, ATGP1456-50, ATGP1456-100, ATGP1456-250, ATGP1456-500, ATGP1456-1

Koike N., et al. (2000) FEBS Lett. 467:17-21; ; Lachner M., et al. (2001) Nature. 410:116-120;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Epigenomics (Transcription & Translation)

NP_057671

Q13185

Human

MASNKT TLQKMGKKQN GKSKKVEEAE PEEFVVEKVL DRRVVNGKVE YFLKWKGFTD ADNTWEPEEN LDCPELIEAF LNSQKAGKEK DGTKRKSLSD SESDDSKSKK KRDAADKPRG FARGLDPERI IGATDSSGEL MFLMKWKDSD EADLVLAKEA NMKCPQIVIA FYEERLTWHS CPEDEAQ