Recombinant humaan CBX5-eiwit

Chromobox homolog 5, also known as CBX5, is a member of the heterochromatin protein family. The protein is enriched in the heterochromatin and associated with centromeres. CBX5 is involved in the formation of functional kinetochore through interaction with essential kinetochore proteins. It has a pseudogene located on chromosome 3. Also, The CBX5 proteins reassociate with chromatin at the end of mitosis, as Histone H3 is dephosphorylated. Recombinant human CBX5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Chromobox homolog 5, HP1, HP1A

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

24.8 kDa (215aa) confirmed by MALDI-TOF

CBX5, ATGP1461-10 µg, ATGP1461-20 µg, ATGP1461-50 µg, ATGP1461-100 µg, ATGP1461-250 µg, ATGP1461-500 µg, ATGP1461-1 mg, ATGP1461-10, ATGP1461-20, ATGP1461-50, ATGP1461-100, ATGP1461-250, ATGP1461-500, ATGP1461-1

Koike N., et al. (2000) FEBS Lett. 467:17-21.; ; Verreault A., et al. (1996) Cell. 87:95-104

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl

Epigenomics (Transcription & Translation)

NP_001120794

P45973

Human

MGKKTK RTADSSSSED EEEYVVEKVL DRRVVKGQVE YLLKWKGFSE EHNTWEPEKN LDCPELISEF MKKYKKMKEG ENNKPREKSE SNKRKSNFSN SADDIKSKKK REQSNDIARG FERGLEPEKI IGATDSCGDL MFLMKWKDTD EADLVLAKEA NVKCPQIVIA FYEERLTWHA YPEDAENKEK ETAKS