Recombinant humaan CCDC101/SGF29-eiwit

Coiled-coil domain containing 101 (CCDC101), also known as SGF29, has been identified as a subunit of the SAGA (Spt-Ada-Gcn5 acetyltransferase) histone acetyltransferase complex in Saccharomyces cerevisiae, which is conserved from yeast to humans. Recombinant human CCDC101 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Coiled-coil domain containing 101, SGF29, STAF36

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

35.4 kDa (313aa) confirmed by MALDI-TOF

CCDC101, Coiled-coil domain containing 101, SGF29, STAF36, ATGP1462-10 µg, ATGP1462-20 µg, ATGP1462-50 µg, ATGP1462-100 µg, ATGP1462-250 µg, ATGP1462-500 µg, ATGP1462-1 mg, ATGP1462-10, ATGP1462-20, ATGP1462-50, ATGP1462-100, ATGP1462-250, ATGP1462-500, ATGP1462-1

Bian C., et al. (2011) EMBO J. 30 (14) :2829-42; ; LiJ., et al. (2010) Acta Crystallogr Sect F Struct Biol Cryst Commun. 66:902-4.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Epigenomics (Transcription & Translation)

NP_612423

Q96ES7

Human

MALVSADSRI AELLTELHQL IKQTQEERSR SEHNLVNIQK THERMQTENK ISPYYRTKLR GLYTTAKADA EAECNILRKA LDKIAEIKSL LEERRIAAKI AGLYNDSEPP RKTMRRGVLM TLLQQSAMTL PLWIGKPGDK PPPLCGAIPA SGDYVARPGD KVAARVKAVD GDEQWILAEV VSYSHATNKY EVDDIDEEGK ERHTLSRRRV IPLPQWKANP ETDPEALFQK EQLVLALYPQ TTCFYRALIH APPQRPQDDY SVLFEDTSYA DGYSPPLNVA QRYVVACKEP KKK