Recombinant humaan CCL14/HCC-1 eiwit

CCL14, also known as Chemokine (C-C motif) ligand 14, is member of a superfamily of inducible, secreted, proinflammatory cytokines. It has weak activities on human monocytes and acts via receptors that also recognize MIP-1alpha. It also enhances the proliferation of CD34 myeloid progenitor cells. The processed form of CCL14, designated HCC-1 (9-74), is a chemotactic factor that attracts monocytes, eosinophils and T cells and is a ligand for CCR1, CCR3 and CCR5. Recombinant human CCL14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

SY14, Small-inducible cytokine A14, SCYL2, SCYA14, NCC-2, NCC2, MCIF, HCC-3, HCC-1, FLJ16015, CKb1, Chemokine CC-1/CC-3, Chemokine (C-C motif) ligand 14, CC-3, CC-1, C-C motif chemokine 14

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

10.9 kDa (95aa) confirmed by MALDI-TOF

CCL14a, HCC1, C-C motif chemokine 14, CC-1, CC-3, CKb1, FLJ16015, HCC-1, HCC-3, MCIF, NCC-2, NCC2, SCYA14, SCYL2, SY14, Chemokine (C-C motif) ligand 14, ATGP0930-10 µg, ATGP0930-20 µg, ATGP0930-50 µg, ATGP0930-100 µg, ATGP0930-250 µg, ATGP0930-500 µg, ATGP0930-1 mg, ATGP0930-10, ATGP0930-20, ATGP0930-50, ATGP0930-100, ATGP0930-250, ATGP0930-500, ATGP0930-1

Munch J., et al. (2002) Antimicrob Agents Chemother. 46:982-990.; ; Blain K.Y., et al. (2007) Biochemistry. 46:10008-10015.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_116739

Q16627

Human

MGSSHHHHHH SSGLVPRGSH MTKTESSSRG PYHPSECCFT YTTYKIPRQR IMDYYETNSQ CSKPGIVFIT KRGHSVCTNP SDKWVQDYIK DMKEN