Recombinant humaan CCL1/I-309 eiwit

CCL1 (chemokine C-C motif ligand 1) is a small glycoprotein secreted by activated T cells that belongs to a family inflammatory cytokines known as chemokines. CCL1 attracts monocytes, NK cells, and immature B cells and dendritic cells by interacting with a cell surface chemokine receptor called CCR8. unlike the other members of the C-C family, CCL1 does not induce chemotaxis in natural killer (NK) cells. Recombinant human CCL1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

C-C motif chemokine 1, Chemokine (C-C motif) ligand 1, Small-inducible cytokine A1, T lymphocyte-secreted protein I-309, inflammatory cytokine I-309, I-309 homologous to mouse Tca-3, SCYA1, TCA3, P500, SISe

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

10.8 kDa (94aa) confirmed by MALDI-TOF

CCL1, I309, Chemokine C-C motif ligand 1, I-309, P500, SCYA1, SISe, TCA3, ATGP0947-10 µg, ATGP0947-20 µg, ATGP0947-50 µg, ATGP0947-100 µg, ATGP0947-250 µg, ATGP0947-500 µg, ATGP0947-1 mg, ATGP0947-10, ATGP0947-20, ATGP0947-50, ATGP0947-100, ATGP0947-250, ATGP0947-500, ATGP0947-1

Miller M.D., et al. (1992) Proc. Natl. Acad. Sci. u.S.A. 89:2950-2954 ; ; Roos RS, et al. (1997) J. Biol. Chem. 272 (28) : 17251-4.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 2mM DTT, 10% glycerol, 50 mM NaCl

Hormones & Cytokines

NP_002972

P22362

Human

MGSSHHHHHH SSGLVPRGSH MKSMQVPFSR CCFSFAEQEI PLRAILCYRN TSSICSNEGL IFKLKRGKEA CALDTVGWVQ RHRKMLRHCP SKRK