Recombinant humaan CCL20/MIP-3 alfa-eiwit

CCL20, also known as Macrophage Inflammatory Protein-3 (MIP3A), is a small cytokine belonging to the CC chemokine family. CCL20 protein is strongly chemotactic for lymphocytes and weakly attracts neutrophils. It is implicated in the formation and function of mucosal lymphoid tissues via chemoattraction of lymphocytes and dendritic cells towards the epithelial cells surrounding these tissues. Recombinant human CCL20 protein, fused to His-tag at N-terminus, was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

C-C motif chemokine ligand 20, Beta-chemokine exodus-1, CC chemokine LARC, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP-3-alpha, MIP-3a, Small-inducible cytokine A20, SCYA20, ST38, CKb4

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

10.3 kDa (91aa) confirmed by MALDI-TOF

CCL20, MIP3 a, ATGP0917-10 µg, ATGP0917-20 µg, ATGP0917-50 µg, ATGP0917-100 µg, ATGP0917-250 µg, ATGP0917-500 µg, ATGP0917-1 mg, ATGP0917-10, ATGP0917-20, ATGP0917-50, ATGP0917-100, ATGP0917-250, ATGP0917-500, ATGP0917-1

Hieshima K, et al. (1997) J Biol Chem. 272:5846-53.; ; Schutyser E, et al. (2000) J. Immunol. 165:4470-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_004582

P78556

Human

MGSSHHHHHH SSGLVPRGSH MASNFDCCLG YTDRILHPKF IVGFTRQLAN EGCDINAIIF HTKKKLSVCA NPKQTWVKYI VRLLSKKVKN M