Recombinant humaan CCL25/TECK-eiwit

CCL25 also known as C-C motif chemokine 25 isoform 1. CCL25 is potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. It binds to CCR9. Isoform 2 is an antagonist of isoform1 and binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4. Recombinant human CCL25-iso1, fused to His-tag at N-terminus, was expressed in E. coli.

C-C motif chemokine ligand 25, Chemokine TECK, Small-inducible cytokine A25, SCYA25, Thymus-expressed chemokine, TECKvar, Ckb15, Ck beta-15

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

16.8 kDa (152aa)

CCL25, TECK, C-C motif chemokine 25 isoform 1, Ckb15, SCYA25, TECK, ATGP2997-10 µg, ATGP2997-20 µg, ATGP2997-50 µg, ATGP2997-100 µg, ATGP2997-250 µg, ATGP2997-500 µg, ATGP2997-1 mg, ATGP2997-10, ATGP2997-20, ATGP2997-50, ATGP2997-100, ATGP2997-250, ATGP2997-500, ATGP2997-1

Watts A.O. et al. (2013) J. Biol. Chem. 288:7169-7181.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Immunology

NP_005615

O15444

Human

QGVFE DCCLAYHYPI GWAVLRRAWT YRIQEVSGSC NLPAAIFYLP KRHRKVCGNP KSREVQRAMK LLDARNKVFA KLHHNTQTFQ AGPHAVKKLS SGNSKLSSSK FSNPISSSKR NVSLLISANS GL