Recombinant humaan CCL3/MIP-1-alfa-eiwit

CCL3, also known as, macrophage inflammatory protein-1alpha (MIP-1alpha), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. This protein has been identified as one of the major HIV-SFs produced by CD8+ T cells, along with MIP-1beta and RANTES. It acts as an inhibitor of different strains of HIV-1, HIV-2 and SIV infection in a dose-dependent manner. Recombinant human CCL3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purifed by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Tonsillar lymphocyte LD78 alpha protein, Small-inducible cytokine A3, SIS-beta, SCYA3, PAT 464.1, MIP-1-alpha, Macrophage inflammatory protein 1-alpha, LD78ALPHA, G0S19-1, G0/G1 switch regulatory protein 19-1, C-C motif chemokine ligand 3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

10 kDa (90aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

CCL3, MIP1 a, C-C motif chemokine 3, G0S19-1, LD78ALPHA. MIP-1-alpha. MIP1A. SCYA3, ATGP1019-10 µg, ATGP1019-20 µg, ATGP1019-50 µg, ATGP1019-100 µg, ATGP1019-250 µg, ATGP1019-500 µg, ATGP1019-1 mg, ATGP1019-10, ATGP1019-20, ATGP1019-50, ATGP1019-100, ATGP1019-250, ATGP1019-500, ATGP1019-1

Guan E, et al. (2001) J Biol Chem. 276:12404-9; ; Cocchi F., et al. (1995) Science. 270:1811-5.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 10 mM Sodium Citrate buffer (pH 3.5) containing 20% glycerol

Hormones & Cytokines

NP_002974.1

P10147

Human

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