Recombinant humaan CCM2-eiwit

CCM2 provides instructions for making a protein called malcavernin, which strengthens the interactions between cells that form blood vessels and limits leakage from the vessels. It interacts with a number of other proteins to form a complex that is found in the junctions that connect neighboring cells. It helps turn off (suppress) a signaling molecule known as RhoA-GTPase. Recombinant human CCM2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Malcavernin isoform 4, Malcavernin isoform 4, Malcavernin isoform 4

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.3 kDa (311aa) confirmed by MALDI-TOF

CCM2, Malcavernin isoform 4, Malcavernin isoform 4, Malcavernin isoform 4, ATGP2496-10 µg, ATGP2496-20 µg, ATGP2496-50 µg, ATGP2496-100 µg, ATGP2496-250 µg, ATGP2496-500 µg, ATGP2496-1 mg, ATGP2496-10, ATGP2496-20, ATGP2496-50, ATGP2496-100, ATGP2496-250, ATGP2496-500, ATGP2496-1

Dupre N. et al. (2003) Can J Neurol Sci. 30:122-128; ; Liquori CL. et al. (2007) Am J Hum Genet. 80:69-75.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Signal Transduction

NP_001161407

Q9BSQ5

Human

EVKYLGQ LTSIPGYLNP SSRTEILHFI DNAKRAHQLP GHLTQEHDAV LSLSAYNVKL AWRDGEDIIL RVPIHDIAAV SYVRDDAAHL VVLKTDDSST KVDIKETYEV EASTFCFPES VDVGGASPHS KTISESELSA SATELLQDYM LTLRTKLSSQ EIQQFAALLH EYRNGASIHE FCINLRQLYG DSRKFLLLGL RPFIPEKDSQ HFENFLETIG VKDGRGIITD SFGRHRRALS TTSSSTTNGN RATGSSDDRS APSEGDEWDR MISDISSDIE ALGCSMDQDS A