Recombinant humaan CD14-eiwit

CD14 is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide. Alternative splicing results in multiple transcript variants encoding the same protein. Recombinant human CD14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, CD14 molecule

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.9 kDa (353aa) confirmed by MALDI-TOF

CD14, monocyte differentiation antigen CD14, Myeloid cell-specific leucine-rich glycoprotein, ATGP2697-10 µg, ATGP2697-20 µg, ATGP2697-50 µg, ATGP2697-100 µg, ATGP2697-250 µg, ATGP2697-500 µg, ATGP2697-1 mg, ATGP2697-10, ATGP2697-20, ATGP2697-50, ATGP2697-100, ATGP2697-250, ATGP2697-500, ATGP2697-1

Kelley S.L., et al (2013) . J. Immunol. 190:1304-1311; ; Setoguchi M., et al (1989) . Biochim. Biophys. Acta 1008:213-222;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 30% glycerol 0.15M NaCl, 1mM DTT

Immunology

NP_001167576

P08571

Human

MGSSHHHHHH SSGLVPRGSH MGSTTPEPCE LDDEDFRCVC NFSEPQPDWS EAFQCVSAVE VEIHAGGLNL EPFLKRVDAD ADPRQYADTV KALRVRRLTV GAAQVPAQLL VGALRVLAYS RLKELTLEDL KITGTMPPLP LEATGLALSS LRLRNVSWAT GRSWLAELQQ WLKPGLKVLS IAQAHSPAFS CEQVRAFPAL TSLDLSDNPG LGERGLMAAL CPHKFPAIQN LALRNTGMET PTGVCAALAA AGVQPHSLDL SHNSLRATVN PSAPRCMWSS ALNSLNLSFA GLEQVPKGLP AKLRVLDLSC NRLNRAPQPD ELPEVDNLTL DGNPFLVPGT ALPHEGSMNS GVV