Recombinant humaan CD146/MCAM-eiwit

MCAM, also known as cell surface glycoprotein MUC18, is an integral membrane glycoprotein belonging to the immunoglobulin superfamily. It is associated with various carcinomas such as tumor progression, metastasis and may be involved in embryonic neural development. This protein also plays a role in cell adhesion, and in cohesion of the endothelial monolayer at intercellular junctions in vascular tissue. Recombinant human MCAM protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Melanoma cell adhesion molecule, Cell surface glycoprotein MUC18, Cell surface glycoprotein P1H12, Melanoma-associated antigen A32, Gicerin, Melanoma-associated antigen MUC18, MUC18, S-endo 1 endothelial-associated antigen, CD146, MelCAM, METCAM, HEMCAM

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

61kDa (547aa)

MCAM, Cell surface glycoprotein MUC18, MCAM, CD146, MUC18, ATGP3715-10 µg, ATGP3715-20 µg, ATGP3715-50 µg, ATGP3715-100 µg, ATGP3715-250 µg, ATGP3715-500 µg, ATGP3715-1 mg, ATGP3715-10, ATGP3715-20, ATGP3715-50, ATGP3715-100, ATGP3715-250, ATGP3715-500, ATGP3715-1

Dagur PK., et al, (2014) Clin Immunol. 152:36-47.; ; Wragg JW., et al, (2016) Cancer Res. 76:2314-2326.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_006491

P43121

Human

Val24

VPGEAEQPAP ELVEVEVGST ALLKCGLSQS QGNLSHVDWF SVHKEKRTLI FRVRQGQGQS EPGEYEQRLS LQDRGATLAL TQVTPQDERI FLCQGKRPRS QEYRIQLRVY KAPEEPNIQV NPLGIPVNSK EPEEVATCVG RNGYPIPQVI WYKNGRPLKE EKNRVHIQSS QTVESSGLYT LQSILKAQLV KEDKDAQFYC ELNYRLPSGN HMKESREVTV PVFYPTEKVW LEVEPVGMLK EGDRVEIRCL ADGNPPPHFS ISKQNPSTRE AEEETTNDNG VLVLEPARKE HSGRYECQGL DLDTMISLLS EPQELLVNYV SDVRVSPAAP ERQEGSSLTL TCEAESSQDL EFQWLREETG QVLERGPVLQ LHDLKREAGG GYRCVASVPS IPGLNRTQLV NVAIFGPPWM AFKERKVWVK ENMVLNLSCE ASGHPRPTIS WNVNGTASEQ DQDPQRVLST LNVLVTPELL ETGVECTASN DLGKNTSILF LELVNLTTLT PDSNTTTGLS TSTASPHTRA NSTSTERKLP EPESRGAAAL EHHHHHH