Recombinant humaan CD147/BSG-eiwit

BSG, also known as basigin isoform 2, is a type 1 transmembrane protein with various different N- and O- glycosylation that belongs to the immunoglobulin superfamily. As members of the immunoglobulin superfamily play fundamental roles in intercellular recognition involved in various immunologic phenomena, differentiation, and development, it is thought also to play a role in intercellular recognition. This protein has many ligands, including the cyclophilin (CyP) proteins Cyp-A and CyP-B and certain integrins and regulates several distinct functions, such as spermatogenesis, expression of the mono-carboxylate transporter and the responsiveness of lymphocytes. Also, it induces the production and release of matrix metalloproteinases in the surrounding mesenchymal cells and tumor cells, and thereby promotes invasion, metastasis, growth and survival of malignant cells. Recombinant human BSG, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Basigin isoform 2, OK, Ok blood group, Extracellular matrix metalloproteinase inducer, Tumor cell-derived collagenase stimulatory factor, TCSF, Collagenase stimulatory factor, Hepatoma-associated antigen, HAb18G, Leukocyte activation antigen M6, EMMPRIN, CD147, EMPRIN, 5F7

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

47.5 kDa (428aa)

BSG, Basigin isoform 2, BSG, 5F7, CD147, EMMPRIN, OK, TCSF, ATGP3737-10 µg, ATGP3737-20 µg, ATGP3737-50 µg, ATGP3737-100 µg, ATGP3737-250 µg, ATGP3737-500 µg, ATGP3737-1 mg, ATGP3737-10, ATGP3737-20, ATGP3737-50, ATGP3737-100, ATGP3737-250, ATGP3737-500, ATGP3737-1

Gabison EE., et al, (2005) Biochimie 87:361-368.; ; Hanna SM., et al, (2003) BMC Biochem 4:17-25.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_940991

P35613

Human

Ala

ADPAAGTVFT TVEDLGSKIL LTCSLNDSAT EVTGHRWLKG GVVLKEDALP GQKTEFKVDS DDQWGEYSCV FLPEPMGTAN IQLHGPPRVK AVKSSEHINE GETAMLVCKS ESVPPVTDWA WYKITDSEDK ALMNGSESRF FVSSSQGRSE LHIENLNMEA DPGQYRCNGT SSKGSDQAII TLRVRSHLAL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH