Recombinant humaan CD158d/KIR2DL4-eiwit

KIR2DL4, also known as killer cell immunoglobulin-like receptor 2DL4, is a member of the killer cell Ig-like receptor (KIR) family. KIR proteins with the long cytoplasmic domain transduce inhibitory signals via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. This protein induces NK cells to produce IFN-gamma and stimulation with IL-2 upregulates cell surface expression on CD56dim cells and leads to the inhibition of the cytolytic NK cell function. Recombinant human KIR2DL4 protein, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Killer cell immunoglobulin like receptor two Ig domains and long cytoplasmic tail 4, Killer cell immunoglobulin-like receptor 2DL4, CD158 antigen-like family member D, G9P, Killer cell inhibitory receptor 103AS, KIR-103AS, MHC class I NK cell receptor KIR103AS, CD158D, KIR103AS,103AS, 15.212

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

51kDa (458aa)

KIR2DL4, CD158d, killer cell immunoglobulin-like receptor 2DL4, KIR2DL4, CD158D, G9P, KIR-103AS, KIR-2DL4, KIR103, KIR103AS, ATGP3639-10 µg, ATGP3639-20 µg, ATGP3639-50 µg, ATGP3639-100 µg, ATGP3639-250 µg, ATGP3639-500 µg, ATGP3639-1 mg, ATGP3639-10, ATGP3639-20, ATGP3639-50, ATGP3639-100, ATGP3639-250, ATGP3639-500, ATGP3639-1

Le Page ME., et al, (2014) J Immunol. 192:732-740.; ; Li G., et al, (2008) J Leukoc Biol. 84:824-834.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_002246

Q99706

Human

His24

HVGGQDKPFC SAWPSAVVPQ GGHVTLRCHY RRGFNIFTLY KKDGVPVPEL YNRIFWNSFL ISPVTPAHAG TYRCRGFHPH SPTEWSAPSN PLVIMVTGLY EKPSLTARPG PTVRAGENVT LSCSSQSSFD IYHLSREGEA HELRLPAVPS INGTFQADFP LGPATHGETY RCFGSFHGSP YEWSDPSDPL PVSVTGNPSS SWPSPTEPSF KTGIARHLHL EPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GKHHHHHH