Recombinant humaan CD16b/FCGR3B-eiwit

FCGR3B, also known as low affinity immunoglobulin gamma Fc region receptor III-B isoform 2, is a member of Ig super family. It encodes a glycosylphosphatidylinositol (GPI) -anchored protein that is expressed constitutively by neutrophils and after gamma-interferon stimulation by eosinophils. This protein binds to complex or aggregated IgG and monomeric IgG. Unlike III-A, it cannot mediate antibody-dependent cytotoxicity and phagocytosis. It can be a trap of immune complexes in peripheral blood circulation that does not activate neutrophils. Recombinant human FCGR3B, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Low affinity immunoglobulin gamma Fc region receptor III-B isoform 2, IgG Fc receptor III-1, IGFR3, FCRIIIb, FCRIII, FCR-10, FCGR3B, FCGR3, FcgammaRIIIb, Fc-gamma RIII-beta, FCG3, Fc gamma receptor IIIb, Fc fragment of IgG receptor IIIb, CD16-I, CD16b, CD16

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

21.4 kDa (188aa)

Fc g RIIIB, CD16b, Low affinity immunoglobulin gamma Fc region receptor III-B isoform 2, FCGR3B, CD16, CD16b, FCG3, FCGR3, FCR-10, FCRIII, FCRIIIb, ATGP3673-10 µg, ATGP3673-20 µg, ATGP3673-50 µg, ATGP3673-100 µg, ATGP3673-250 µg, ATGP3673-500 µg, ATGP3673-1 mg, ATGP3673-10, ATGP3673-20, ATGP3673-50, ATGP3673-100, ATGP3673-250, ATGP3673-500, ATGP3673-1

Ravetch J.V., et al. (2001) Annu. Rev. Immunol. 19:275-290.; ; Machado LR., et al. (2012) Am. J. Hum. Genet. 90:973-985.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_000561.3

O75015

Human

Glu21