Recombinant humaan CD1b-eiwit

CD1B is a member of the CD1 family of transmembrane glycoproteins, which are structurally related to the major histocompatibility complex (MHC) proteins and form heterodimers with beta-2-microglobulin. This protein contains 1 Ig-like (immunoglobulin-like) domain. CD1B is antigen-presenting protein that binds self and non-self lipid and glycolipid antigens and presents them to T-cell receptors on natural killer T-cells. Recombinant human CD1B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

T-cell surface glycoprotein CD1b, CD1, CD1A, R1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

34.2 kDa (310aa) confirmed by MALDI-TOF

CD1b, T-cell surface glycoprotein CD1b, CD1, CD1A, R1, ATGP1783-10 µg, ATGP1783-20 µg, ATGP1783-50 µg, ATGP1783-100 µg, ATGP1783-250 µg, ATGP1783-500 µg, ATGP1783-1 mg, ATGP1783-10, ATGP1783-20, ATGP1783-50, ATGP1783-100, ATGP1783-250, ATGP1783-500, ATGP1783-1

Shamshiev A., et al. (2000) Immunity 13:255-264 ; ; Winau F., et al. (2004) Nat. Immunol. 5:169-174;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 20% glycerol,1mM DTT

Immunology

NP_001755

P29016

Human

SEHAFQ GPTSFHVIQT SSFTNSTWAQ TQGSGWLDDL QIHGWDSDSG TAIFLKPWSK GNFSDKEVAE LEEIFRVYIF GFAREVQDFA GDFQMKYPFE IQGIAGCELH SGGAIVSFLR GALGGLDFLS VKNASCVPSP EGGSRAQKFC ALIIQYQGIM ETVRILLYET CPRYLLGVLN AGKADLQRQV KPEAWLSSGP SPGPGRLQLV CHVSGFYPKP VWVMWMRGEQ EQQGTQLGDI LPNANWTWYL RATLDVADGE AAGLSCRVKH SSLEGQDIIL YWRNPTSIGS