Recombinant humaan CD200R1-eiwit

CD200R1, also known as cell surface glycoprotein CD200 receptor 1 isoform d, is a transmembrane protein in the immunoglobulin superfamily. This glycoprotein contains on C2 type Ig-like domain and V type Ig-like domain within its extracellular domain and a PTB-signaling motif in cytoplasmic domain. Also, it is expressed on myeloid cells and CD4+ T cells. For function, this receptor is restricted to the surfaces of myeloid lineage cells and the receptor-substrate interaction may function as a myeloid down regulatory signal. Activation of CD200R1 regulates the expression of inflammatory molecules such as tumor necrosis factor (TNF-alpha), interferon and inducible nitric oxide synthase (iNOS) . Recombinant human CD200R1, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

CD200 (ATGP4013) is coated at 10 µg/mL (100 ul/well) can bind Human CD200R1. The ED50 range ≤ 1 µg/mL.

Cell surface glycoprotein CD200 receptor 1 isoform d, CD200 cell surface glycoprotein receptor, Cell surface glycoprotein OX2 receptor 1, CD200R, CRTR2, MOX2 receptor, MOX2R, OX2R, HCRTR2

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human CD200 (CAT# ATGP4013) . The ED50 range ≤ 1 µg/mL.

51.9 kDa (462aa)

CD200R1, Cell surface glycoprotein CD200 receptor 1 isoform d, CD200R1, CD200R, HCRTR2, MOX2R, OX2R, ATGP3809-10 µg, ATGP3809-20 µg, ATGP3809-50 µg, ATGP3809-100 µg, ATGP3809-250 µg, ATGP3809-500 µg, ATGP3809-1 mg, ATGP3809-10, ATGP3809-20, ATGP3809-50, ATGP3809-100, ATGP3809-250, ATGP3809-500, ATGP3809-1

Wright GJ., et al, (2003) J. Immunol. 171:3034-3046.; ; Liu JQ., et al, (2016) J. Immunol. 197:1489-1497.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_740750

Q8TD46

Human

Ala

SSSLCMD EKQITQNYSK VLAEVNTSWP VKMATNAVLC CPPIALRNLI IITWEIILRG QPSCTKAYKK ETNETKETNC TDERITWVSR PDQNSDLQIR TVAITHDGYY RCIMVTPDGN FHRGYHLQVL VTPEVTLFQN RNRTAVCKAV AGKPAAHISW IPEGDCATKQ EYWSNGTVTV KSTCHWEVHN VSTVTCHVSH LTGNKSLYIE LLPVPGAKKS AKL