Recombinant humaan CD27 Ligand/CD70 eiwit

CD70, also known as CD70 antigen, is a transmembrane protein in the TNF receptor superfamily. It functions as a co-stimulatory molecule that supports lymphocyte activation and survival. This protein binds to the transmembrane glycoprotein CD27 ligand/CD70 which is expressed on activated B cells, activated T cells, and dendritic cells. Binding of CD70 to its receptor CD27 induces in priming, effector functions, differentiation and memory formation of T cell activation, and thus is proliferation of co-stimulated T cells, as well as the generation of cytolytic T cells. Recombinant human CD70, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human CD27 Ligand/TNFSF7 is coated at 10 µg/mL (100 ul/well) can bind Human CD27/TNFRSF7 (CAT# ATGP3416) in a Functional ELISA assay.

CD70 antigen, CD27L, CD27LG, TNFSF7, tumor necrosis factor superfamily member 7

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human CD27/TNFRSF7 (CAT# ATGP3416) .

18.2 kDa (164aa)

CD27 Ligand, TNFSF7, CD70 antigen, CD27L, CD27LG, TNFSF7, ATGP3336-10 µg, ATGP3336-20 µg, ATGP3336-50 µg, ATGP3336-100 µg, ATGP3336-250 µg, ATGP3336-500 µg, ATGP3336-1 mg, ATGP3336-10, ATGP3336-20, ATGP3336-50, ATGP3336-100, ATGP3336-250, ATGP3336-500, ATGP3336-1

Welten SP., et al. (2013) J. Virol. 87:6851-6865.; ; Polak ME., et al. (2012) J. Invest. Dermatol. 132:1636-1644.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Hormones & Cytokines

NP_001243.1

P32970

Human

Ala

QRFAQAQ QQLPLESLGW DVAELQLNHT GPQQDPRLYW QGGPALGRSF LHGPELDKGQ LRIHRDGIYM VHIQVTLAIC SSTTASRHHP TTLAVGICSP ASRSISLLRL SFHQGCTIAS QRLTPLARGD TLCTNLTGTL LPSRNTDETF FGVQWVRP