Recombinant humaan CD3-epsiloneiwit

CD3E is the CD3-epsilonpolypeptide, which together with CD3-gamma, -delta and -zeta, and the T-cell receptor alpha/beta and gamma/delta heterodimers, forms the T-cell receptor-CD3 complex. This complex plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. The genes encoding the epsilon, gamma delta polypeptides are located in the same cluster on chromosome 11. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women. Recombinant human CD3E protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

14.1 kDa (127aa) confirmed by MALDI-TOF

CD3 epsilon, ATGP1853-10 µg, ATGP1853-20 µg, ATGP1853-50 µg, ATGP1853-100 µg, ATGP1853-250 µg, ATGP1853-500 µg, ATGP1853-1 mg, ATGP1853-10, ATGP1853-20, ATGP1853-50, ATGP1853-100, ATGP1853-250, ATGP1853-500, ATGP1853-1

Lu, X., et al. (2011) Hematology 16 (3), 185-189; ; Li, Y., et al. (2011) Hematology 16 (3), 143-150;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 10% glycerol

Immunology

NP_000724.1

P07766

Human

DGNEEMG GITQTPYKVS ISGTTVILTC PQYPGSEILW QHNDKNIGGD EDDKNIGSDE DHLSLKEFSE LEQSGYYVCY PRGSKPEDAN FYLYLRARVC ENCMEMD