Recombinant humaan CD3 zeta/CD247 eiwit

T-cell surface glycoprotein CD3 zeta chain, also known as CD247, belongs to the CD3Z/FCER1G family. CD247 is T-cell receptor zeta, which together with T-cell receptor alpha/beta and gamma/delta heterodimers, and with CD3-gamma, -delta and -epsilon, forms the T-cell receptor-CD3 complex. The zeta chain plays an important role in coupling antigen recognition to several intracellular signal-transduction pathways. Low expression of the antigen results in impaired immune response. Recombinant human CD247 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

TCRZ, T-cell surface glycoprotein CD3 zeta chain isoform 1, T3Z, IMD25, CD3-ZETA, CD3Z, CD3Q, CD3H, CD247

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

15.4 kDa (136aa) confirmed by MALDI-TOF

TCRZ, T-cell surface glycoprotein CD3 zeta chain isoform 1, T3Z, IMD25, CD3-ZETA, CD3Z, CD3Q, CD3H, CD247, ATGP1872-10 µg, ATGP1872-20 µg, ATGP1872-50 µg, ATGP1872-100 µg, ATGP1872-250 µg, ATGP1872-500 µg, ATGP1872-1 mg, ATGP1872-010, ATGP1872-020, ATGP1872-050, ATGP1872-100, ATGP1872-250, ATGP1872-500, ATGP1872-01M

Mayya V., et al. (2009) Sci. Signal. 2:RA46-RA46; ; Rieux-Laucat F., et al. (2006) N. Engl. J. Med. 354:1913-1921;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Immunology

NP_932170.1

P20963

Human

RVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPR