Recombinant humaan CD3 zeta/CD247 eiwit

CD247, also known as T-cell surface glycoprotein CD3 zeta chain isoform 1, belongs to CD3Z / FCER1G family. The T cell receptor Zeta together, with alpha / beta and gamma / delta heterodimers. This Protein plays an important role in binding antigen recognition to multiple intracellular signaling pathways and also plays a role in assembly and expression of the TCR complex. Defects in CD247 defects can cause immune deficiencies. Immune deficiency, characterized by T cells, impaired immune responses to allogeneic antigens, tetanus toxoid and mitogen. Recombinant human CD247, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

TCRZ, T-cell surface glycoprotein CD3 zeta chain isoform 1, T3Z, IMD25, CD3-ZETA, CD3Z, CD3Q, CD3H, CD247

Baculovirus

Human

His-Tag

SDS-PAGE

0.2 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

14.1 kDa (122aa)

TCRZ, T-cell surface glycoprotein CD3 zeta chain isoform 1, T3Z, IMD25, CD3-ZETA, CD3Z, CD3Q, CD3H, CD247, ATGP3754-10 µg, ATGP3754-20 µg, ATGP3754-50 µg, ATGP3754-100 µg, ATGP3754-250 µg, ATGP3754-500 µg, ATGP3754-1 mg, ATGP3754-010, ATGP3754-020, ATGP3754-050, ATGP3754-100, ATGP3754-250, ATGP3754-500, ATGP3754-01M

Tomita K., et al. (2001) J. Biol. Chem. 276:25378-25385.; ; Li R., et al. (2012) Lupus. 21:75-83.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 6.8) containing 50% glycerol, 1mM DTT, 1mM EDTA, 0.1M NaCl

Immunology

NP_932170.1

P20963

Human

Ala

RVKFSRS ADAPAYQQGQ NQLYNELNLG RREEYDVLDK RRGRDPEMGG KPQRRKNPQE GLYNELQKDK MAEAYSEIGM KGERRRGKGH DGLYQGLSTA TKDTYDALHM QALPPR