Recombinant humaan CD300A-eiwit

CD300A belongs to the CD300 family and contains 1 Ig-like V-type (immunoglobulin-like) domain. The protein is expressed not only by natural killer (NK) cells but also by T-cell subsets, B-cells, dendritic cells, mast cells, granulocytes and monocytes. CD300A is inhibitory receptor which may contribute to the down-regulation of cytolytic activity in natural killer (NK) cells, and to the down-regulation of mast cell degranulation. Recombinant human CD300A protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CD300a molecule, CMRF35-like molecule 8, CLM-8, CD300 antigen-like family member A, CMRF-35-H9, CMRF35-H9, CMRF35-H, IRC1/IRC2, Immunoglobulin superfamily member 12, IgSF12, Inhibitory receptor protein 60, IRp60, NK inhibitory receptor

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

14.7 kDa (134aa) confirmed by MALDI-TOF

CD300a, LMIR1, CD300a molecule, CLM-8, CMRF-35-H9, CMRF-35H, CMRF35-H, CMRF35-H9, CMRF35H, CMRF35H9, IGSF12, IRC1, IRC1/IRC2, IRC2, IRp60, ATGP2232-10 µg, ATGP2232-20 µg, ATGP2232-50 µg, ATGP2232-100 µg, ATGP2232-250 µg, ATGP2232-500 µg, ATGP2232-1 mg, ATGP2232-10, ATGP2232-20, ATGP2232-50, ATGP2232-100, ATGP2232-250, ATGP2232-500, ATGP2232-1

Clark G.J., et al. (2000) Tissue Antigens. 55:101-109; ; Bachelet I., et al. (2005) J. Immunol. 175:7989-7995

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Immunology

NP_009192

Q9UGN4

Human

LSKCRTV AGPVGGSLSV QCPYEKEHRT LNKYWCRPPQ IFLCDKIVET KGSAGKRNGR VSIRDSPANL SFTVTLENLT EEDAGTYWCG VDTPWLRDFH DPVVEVEVSV FPAS