Recombinant humaan CD31/PECAM1-eiwit

PECAM1, also known as platelet endothelial cell adhesion molecule, induces susceptibility to atherosclerosis. It prevents phagocyte ingestion of closely apposed viable cells by transmitting detachment signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes. The encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. Recombinant human PECAM1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human CD31 stimulates cell adhesion of the Jurkat human acute T cell leukemia cells. This effect is more to 40%.

CD31/EndoCAM, CD31, EndoCAM, GPIIA', PECA1, PECAM1, PECAM-1, Platelet endothelial cell adhesion molecule 1

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When cells are added to human CD31 coated plates 5 µg/mL. This effect is more to 40%.

65.5 kDa (582aa)

CD31, PECAM1, PECAM1, CD31, CD31/EndoCAM, endoCAM, GPIIA', PECA1, PECAM-1, ATGP3214-10 µg, ATGP3214-20 µg, ATGP3214-50 µg, ATGP3214-100 µg, ATGP3214-250 µg, ATGP3214-500 µg, ATGP3214-1 mg, ATGP3214-10, ATGP3214-20, ATGP3214-50, ATGP3214-100, ATGP3214-250, ATGP3214-500, ATGP3214-1

Brown S., et al. (2002) Nature. 418:200-203.; ; Dasgupta B., et al. (2009) J Immunol. 182:5041-5051.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_000433

P16284

Human