Recombinant humaan CD39L3/ENTPD3-eiwit

ENTPD3, also known as ectonucleoside triphosphate diphosphohydrolase 3, is an integral membrane glycoprotein with an extracellular active site. It contains 4 apyrase-conserved regions which is characteristic of NTPases. Recombinant human ENTPD3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 250,000 pmol/min/ug, and is defined as the amount of enzyme that hydrolyze ATP per minute at pH 7.5 at 37C.

Ectonucleoside triphosphate diphosphohydrolase 3, NTPDase 3, CD39 antigen-like 3, Ecto-ATP diphosphohydrolase 3, Ecto-ATPDase 3, Ecto-ATPase 3, Ecto-apyrase 3, HB6

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 250,000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze ATP per minute at pH 7.5 at 37C.

50.7 kDa (451aa)

CD39L3, ENTPD3, Ectonucleoside triphosphate diphosphohydrolase 3, ENTPD3, CD39L3, HB6, NTPDase-3, ATGP3498-10 µg, ATGP3498-20 µg, ATGP3498-50 µg, ATGP3498-100 µg, ATGP3498-250 µg, ATGP3498-500 µg, ATGP3498-1 mg, ATGP3498-10, ATGP3498-20, ATGP3498-50, ATGP3498-100, ATGP3498-250, ATGP3498-500, ATGP3498-1

Lavoie EG., et al. (2004) Biochem. Pharmacol. 67:1917-1926.; ; Fausther M., et al. (2010) Am J Physiol Lung Cell Mol Physiol. 298:L804-818.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Signal Transduction

NP_001239

O75355

Human

Ala

ADLQIHKQEV LPPGLKYGIV LDAGSSRTTV YVYQWPAEKE NNTGVVSQTF KCSVKGSGIS SYGNNPQDVP RAFEECMQKV KGQVPSHLHG STPIHLGATA GMRLLRLQNE TAANEVLESI QSYFKSQPFD FRGAQIISGQ EEGVYGWITA NYLMGNFLEK NLWHMWVHPH GVETTGALDL GGASTQISFV AGEKMDLNTS DIMQVSLYGY VYTLYTHSFQ CYGRNEAEKK FLAMLLQNSP TKNHLTNPCY PRDYSISFTM GHVFDSLCTV DQRPESYNPN DVITFEGTGD PSLCKEKVAS IFDFKACHDQ ETCSFDGVYQ PKIKGPFVAF AGFYYTASAL NLSGSFSLDT FNSSTWNFCS QNWSQLPLLL PKFDEVYARS YCFSANYIYH LFVNGYKFTE ETWPQIHFEK EVGNSSIAWS LGYMLSLTNQ IPAESPLIRL PIEPPHHHHH H