Recombinant humaan CD40 Ligand/CD40LG eiwit

CD40 ligand, also known as CD40LG, is a member of the TNF superfamily and is expressed on activated T cells. CD40LG is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1. Recombinant human CD40LG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CD40 ligand, CD40-L, CD40L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, TNFSF5, CD154, hyper-IgM syndrome, HIGM1, T-B cell-activating molecule, T-BAM

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

18.3 kDa (169aa) confirmed by MALDI-TOF

CD40 Ligand, TNFSF5, CD40 ligand, CD154, CD40L, gp39, hCD40L, HIGM1, IGM, IMD3, T-BAM, TNFSF5, TRAP, ATGP1017-10 µg, ATGP1017-20 µg, ATGP1017-50 µg, ATGP1017-100 µg, ATGP1017-250 µg, ATGP1017-500 µg, ATGP1017-1 mg, ATGP1017-10, ATGP1017-20, ATGP1017-50, ATGP1017-100, ATGP1017-250, ATGP1017-500, ATGP1017-1

Gordon J., et al. (1995) Blood Rev. 9:53-56.; ; Cheng G., et al. (1995) Science. 267:1494-1498.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl

Immunology

NP_000065

P29965

Human

MQKGDQNPQI AAHVISEASS KTTSVLQWAE KGYYTMSNNL VTLENGKQLT VKRQGLYYIY AQVTFCSNRE ASSQAPFIAS LCLKSPGRFE RILLRAANTH SSAKPCGQQS IHLGGVFELQ PGASVFVNVT DPSQVSHGTG FTSFGLLKL