Recombinant humaan CD40/TNFRSF5-eiwit

CD40 is a member of the TNF-receptor superfamily. This receptor has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of this receptor and its ligand, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with this receptor and serves as a mediator of the signal transduction. The interaction of this receptor and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Recombinant human CD40 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, p50, CD40L receptor, CDw40

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

21.6 kDa (196aa) confirmed by MALDI-TOF

CD40, TNFRSF5, CD40 molecule TNF receptor superfamily member 5, CD40 molecule, TNF receptor superfamily member 5, Bp50, CDW40, p50, TNFRSF5, ATGP2615-10 µg, ATGP2615-20 µg, ATGP2615-50 µg, ATGP2615-100 µg, ATGP2615-250 µg, ATGP2615-500 µg, ATGP2615-1 mg, ATGP2615-10, ATGP2615-20, ATGP2615-50, ATGP2615-100, ATGP2615-250, ATGP2615-500, ATGP2615-1

Tone M., et al. (2001) Proc. Natl. Acad. Sci. u.S.A. 98:1751-1756; ; Khandekar S.S., et al. (2001) Protein Expr. Purif. 23:301-310;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Stem cell

NP_001241.1

P25942

Human

EPPTACR EKQYLINSQC CSLCQPGQKL VSDCTEFTET ECLPCGESEF LDTWNRETHC HQHKYCDPNL GLRVQQKGTS ETDTICTCEE GWHCTSEACE SCVLHRSCSP GFGVKQIATG VSDTICEPCP VGFFSNVSSA FEKCHPWTSC ETKDLVVQQA GTNKTDVVCG PQDRLR