Recombinant humaan CD46-eiwit

Membrane cofactor protein isoform 14, also known as CD46, is a type I membrane protein that has cofactor activity for inactivation of complement components C3b and C4b by serum factor I. This function resides in the complement control protein repeats (CCPs), with CCP1-4 essential for regulation. CD46 is widely distributed on thymocytes, T cells, B cells, monocytes, granulocytes, NK cells, platelets, endothelial cells, epithelial cells, fibroblasts, placenta and sperm, but not on erythrocyte. Recombinant human CD46 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Membrane cofactor protein isoform 14, AHuS2, MCP, MIC10, TLX, TRA2.10

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

33.8 kDa (302aa) confirmed by MALDI-TOF

CD46, Membrane cofactor protein isoform 14, AHuS2, MCP, MIC10, TLX, TRA2.10, ATGP2036-10 µg, ATGP2036-20 µg, ATGP2036-50 µg, ATGP2036-100 µg, ATGP2036-250 µg, ATGP2036-500 µg, ATGP2036-1 mg, ATGP2036-10, ATGP2036-20, ATGP2036-50, ATGP2036-100, ATGP2036-250, ATGP2036-500, ATGP2036-1

Riley R C., et al. (2002) Mol Reprod Dev. 62:534-546.; ; Iwata K., et al. (1995) J Biol Chem. 270:15148-15152.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 20% glycerol

Immunology

NP_758860.1

P15529

Human

CEEPPTF EAMELIGKPK PYYEIGERVD YKCKKGYFYI PPLATHTICD RNHTWLPVSD DACYRETCPY IRDPLNGQAV PANGTYEFGY QMHFICNEGY YLIGEEILYC ELKGSVAIWS GKPPICEKVL CTPPPKIKNG KHTFSEVEVF EYLDAVTYSC DPAPGPDPFS LIGESTIYCG DNSVWSRAAP ECKVVKCRFP VVENGKQISG FGKKFYYKAT VMFECDKGFY LDGSDTIVCD SNSTWDPPVP KCLKGPRPTY KPPVSNYPGY PKPEEGILDS LD