Recombinant humaan CD47-eiwit

CD47, also known as leukocyte surface antigen CD47 isoform 1, has a role in both cell adhesions by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It plays an important role in memory formation and synaptic plasticity in the hippocampus. Receptor for SIRPA prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Recombinant human CD47, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human CD47 is coated at 1 µg/mL (100 ul/well) can bind Human SIRP gamma/CD172g (CAT# ATGP4030) in a Functional ELISA assay.

CD47, OA3, IAP, MER6, Leukocyte surface antigen CD47 isoform 1, antigenic surface determinant protein OA3, Integrin-associated protein

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human SIRP gamma/CD172g (CAT# ATGP4030)

14.7 kDa (129aa)

ATGP3188, ATGP4015-10 µg, ATGP4015-20 µg, ATGP4015-50 µg, ATGP4015-100 µg, ATGP4015-250 µg, ATGP4015-500 µg, ATGP4015-1 mg, ATGP4015-10, ATGP4015-20, ATGP4015-50, ATGP4015-100, ATGP4015-250, ATGP4015-500, ATGP4015-1, ATGP4015-10 µg, ATGP4015-20 µg, ATGP4015-50 µg, ATGP4015-100 µg, ATGP4015-250 µg, ATGP4015-500 µg, ATGP4015-1 mg, ATGP4015-10, ATGP4015-20, ATGP4015-50, ATGP4015-100, ATGP4015-250, ATGP4015-500, ATGP4015-1

Iwamoto C et al., (2014) Exp. Hematol. 42 (3) :163-171.; ; Wang Y et al., (2013) Mol. Ther. 21 (10) :1919-1929.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology, Adhesion & Movement

NP_001768

Q08722

Human

Gln19