Recombinant humaan CD55/DAF-eiwit

CD55, also known as complement decay-accelerating factor isoform 1, is a membrane protein that attaches to cell membrane via a glycophosphatidylinositol (GPI) anchor. It contains four complement control protein repeats (CCPs) with a single N-linked glycan positioned between CCPs 1 and 2. It regulates the complement system on the cell surface. It is used as a receptor by some coxsackieviruses and other enteroviruses. It is broadly distributed among hematopoietic and non-hematopoietic cells. It is a determinant for the Cromer blood group system. It plays a role promotion of tumorigenesis, decrease of complement mediated tumor cell lysis, autocrine loops for cell rescue and evasion of apoptosis, neoangiogenesis, invasiveness, cell motility. Recombinant human CD55, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Complement decay-accelerating factor isoform 1, CD55, CHAPLE, CR, CROM, DAF, TC

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by BCA assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

36 kDa (328aa)

CD55, DAF, Complement decay-accelerating factor isoform 1, CD55, CHAPLE, CR, CROM, DAF, TC, ATGP3730-10 µg, ATGP3730-20 µg, ATGP3730-50 µg, ATGP3730-100 µg, ATGP3730-250 µg, ATGP3730-500 µg, ATGP3730-1 mg, ATGP3730-10, ATGP3730-20, ATGP3730-50, ATGP3730-100, ATGP3730-250, ATGP3730-500, ATGP3730-1

Ozen A., et al. (2017) N Engl J Med. 377:52-61.; ; Brodbeck WG., et al. (2000) Immunology. 101:104-111.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_000565

P08174

Human

Ala

ADPDCGLPPD VPNAQPALEG RTSFPEDTVI TYKCEESFVK IPGEKDSVIC LKGSQWSDIE EFCNRSCEVP TRLNSASLKQ PYITQNYFPV GTVVEYECRP GYRREPSLSP KLTCLQNLKW STAVEFCKKK SCPNPGEIRN GQIDVPGGIL FGATISFSCN TGYKLFGSTS SFCLISGSSV QWSDPLPECR EIYCPAPPQI DNGIIQGERD HYGYRQSVTY ACNKGFTMIG EHSIYCTVNN DEGEWSGPPP ECRGKSLTSK VPPTVQKPTT VNVPTTEVSP TSQKTTTKTT TPNAQATRST PVSRTTKHFH ETTPNKGSGT TSHHHHHH