Recombinant humaan CD59-eiwit

CD59 is a cell surface glycoprotein that regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It also plays a role in signal transduction pathways in the activation of T cells. Mutations in this gene cause CD59 deficiency, a disease resulting in hemolytic anemia and thrombosis, and which causes cerebral infarction. Recombinant human CD59 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CD59 glycoprotein, 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

11.3 kDa (100aa) confirmed by MALDI-TOF

CD59, CD59 glycoprotein, 16.3A5, 1F5, EJ16, EJ30, EL32, G344, HRF-20, HRF20, MAC-IP, MACIF, MEM43, MIC11, MIN1, MIN2, MIN3, MIRL, MSK21, p18-20, ATGP2258-10 µg, ATGP2258-20 µg, ATGP2258-50 µg, ATGP2258-100 µg, ATGP2258-250 µg, ATGP2258-500 µg, ATGP2258-1 mg, ATGP2258-10, ATGP2258-20, ATGP2258-50, ATGP2258-100, ATGP2258-250, ATGP2258-500, ATGP2258-1

Ninomiya H., et al (1992) . J. Biol. Chem. 267: 13675-13680 ; ; Rudd P.M., et al (1997) . J. Biol. Chem. 272:7229-7244

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Stem Cell

NP_000602

P13987

Human

MGSSHHHHHH SSGLVPRGSH MGSLQCYNCP NPTADCKTAV NCSSDFDACL ITKAGLQVYN KCWKFEHCNF NDVTTRLREN ELTYYCCKKD LCNFNEQLEN