Recombinant humaan CD74 eiwit

CD74, also known as HLA class II histocompatibility antigen gamma chain or HLA-DR antigens-associated invariant chain or CD74 (Cluster of Differentiation 74) . The nascent MHC class II protein in the rough ER has its peptide-binding cleft blocked by the invariant chain (Ii; a trimer) to prevent it from binding cellular peptides or peptides from the endogenous pathway. The invariant chain also facilitates MHC class II's export from the ER in a vesicle. The signal for endosomal targeting resides in the cytoplasmic tail of the invariant chain. This fuses with a lateendosome containing the endocytosed, degraded proteins. It is then cleaved by cathepsin S (cathepsin L in cortical thymic epithelial cells), leaving only a small fragment calle CLIP which blocks peptide binding until HLA-DM binds to MHC II, releasing CLIP and allowing other peptides to bind. The stable MHC class-II is then presented on the cell surface. Recombinant human CD74, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

HLA class II histocompatibility antigen gamma chain isoform b, DHLAG, HLADG, Ia-GAMMA II

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 85% by SDS-PAGE

19.3kDa (169aa)

CD74, HLA class II histocompatibility antigen gamma chain isoform b, DHLAG, HLADG, Ia-GAMMA II, ATGP3134-10 µg, ATGP3134-20 µg, ATGP3134-50 µg, ATGP3134-100 µg, ATGP3134-250 µg, ATGP3134-500 µg, ATGP3134-1 mg, ATGP3134-10, ATGP3134-20, ATGP3134-50, ATGP3134-100, ATGP3134-250, ATGP3134-500, ATGP3134-1

Claesson L. et al. (1983) Proc Natl Acad Sci U S A. 80 (24) :7395-9.; ; Bakke O. et al. (1990) Cell. 63 (4) :707-16.; ; Strubin M. et al. (1984) EMBO J. 3 (4) :869-72.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_004346.1

P04233

Human

Ala

QQQGRLD KLTVTSQNLQ LENLRMKLPK PPKPVSKMRM ATPLLMQALP MGALPQGPMQ NATKYGNMTE DHVMHLLQNA DPLKVYPPLK GSFPENLRHL KNTMETIDWK VFESWMHHWL LFEMSRHSLE QKPTDAPPKE SLELEDPSSG LGVTKQDLGP VPM