Recombinant humaan CD83-eiwit

CD83 is a single-pass type I membrane protein and member of the immunoglobulin superfamily of receptors. The protein may be involved in the regulation of antigen presentation. A soluble form of this protein can bind to dendritic cells and inhibit their maturation. Three transcript variants encoding different isoforms have been found for this gene. Recombinant human CD83 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

CD83 antigen isoform b precursor, BL11, HB15

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

16 kDa (148aa) confirmed by MALDI-TOF

CD83, CD83 antigen isoform b precursor, BL11, HB15, ATGP1925-10 µg, ATGP1925-20 µg, ATGP1925-50 µg, ATGP1925-100 µg, ATGP1925-250 µg, ATGP1925-500 µg, ATGP1925-1 mg, ATGP1925-10, ATGP1925-20, ATGP1925-50, ATGP1925-100, ATGP1925-250, ATGP1925-500, ATGP1925-1

Zhou LJ, Tedder TF, et al. (1995) . J Immunol. 154 (8) :3821-35.; ; Lechmann M, Berchtold S, et al. (2002) . Trends Immunol. 23 (6) :273-5.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol

Immunology

NP_001035370

Q01151

Human

TPEVKVA CSEDVDLPCT APWDPQVPYT VSWVKLLEGG EERMETPQED HLRGQHYHQK GQNGSFDAPN ERPYSLKIRN TTSCNSGTYR CTLQDPDGQR NLSGKVILRV TGCPAQRKEE TFKKYRAE