Recombinant humaan CD95/FAS-eiwit

FAS, also known as tumor necrosis factor receptor superfamily member 6, belongs to the death receptor subfamily of the TNF receptor superfamily and is designated TNFRSF6. This protein plays a major role in controlling viral infections. While FAS is expressed on most cell types, its cognate ligand (FasL) is restricted to activated T, NK and dendritic cells. The upregulation of FasL and TRAIL on HCMV-infected dendritic cells promotes direct killing of activated T lymphocytes, an action that may preferentially delete HCMV-specific T cells. Moreover, the activation of FasL on HCMVinfected retinal pigment epithelial cells may subvertneutrophil function in HCMV retinitis. Recombinant human FAS, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human CD95/FAS inhibit apoptosis of the Jurkat human acute T cell leukemia cells in the presence of human FAS ligand. The ED50 range ≤ 200 ng/mL.

Tumor necrosis factor receptor superfamily member 6, Apo-1 antigen, Apoptosis-mediating surface antigen FAS, FASLG receptor, FAS1, APT1, TNFRSF6

Baculovirus

Human

His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by ability to inhibit FAS ligand-induced apoptosis assay using Jurkat human acute T cell leukemia cells in the presence of 2 ng/mL of human FAS ligand. The ED50 range ≤ 200 ng/mL.

17.7 kDa (156aa)

Fas, TNFRSF6, CD95, FAS, ALPS1A, APO-1, APT1, CD95, FAS1, FASTM, TNFRSF6, ATGP3267-10 µg, ATGP3267-20 µg, ATGP3267-50 µg, ATGP3267-100 µg, ATGP3267-250 µg, ATGP3267-500 µg, ATGP3267-1 mg, ATGP3267-10, ATGP3267-20, ATGP3267-50, ATGP3267-100, ATGP3267-250, ATGP3267-500, ATGP3267-1

Seirafian S., et al. (2014) J. Gen. Virol. 95 (4) :933-939.; ; Thurner EM., et al. (2014) Strahlenther Onkol 190 (3) :304-309.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Apoptosis

NP_000034

P25445

Human