Recombinant humaan CD99-eiwit

CD99, also known as CD99 antigen isoform a, is a type 1 transmembrane glycoprotein and the founding member of the CD99 family of molecules. Cells known to express this protein include fibroblasts, neutrophils, T cells, CD34+ stem cells, monocytes and endothelial cells. Also, it plays a role in a late step of leukocyte extravasation helping leukocytes to overcome the endothelial basement membrane. Recombinant human CD99, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

CD99 antigen isoform a, CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE

1 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

37.3 kDa (342aa)

CD99, CD99 antigen isoform a, CD99, HBA71, MIC2, MIC2X, MIC2Y, MSK5X, ATGP3615-10 µg, ATGP3615-20 µg, ATGP3615-50 µg, ATGP3615-100 µg, ATGP3615-250 µg, ATGP3615-500 µg, ATGP3615-1 mg, ATGP3615-10, ATGP3615-20, ATGP3615-50, ATGP3615-100, ATGP3615-250, ATGP3615-500, ATGP3615-1

Hahn JH., et al, (1997) J. Immunol. 159:2250-2258.; ; Gil M., et al, (2015) Immunol. Lett. 168:329-336.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_002405

P14209

Human

Ala

ADPDGGFDLS DALPDNENKK PTAIPKKPSA GDDFDLGDAV VDGENDDPRP PNPPKPMPNP NPNHPSSSGS FSDADLADGV SGGEGKGGSD GGGSHRKEGE EADLEPKSCD KTHTCPPCPA PELLGGPSVF LFPPKPKDTL MISRTPEVTC VVVDVSHEDP EVKFNWYVDG VEVHNAKTKP REEQYNSTYR VVSVLTVLHQ DWLNGKEYKC KVSNKALPAP IEKTISKAKG QPREPQVYTL PPSRDELTKN QVSLTCLVKG FYPSDIAVEW ESNGQPENNY KTTPPVLDSD GSFFLYSKLT VDKSRWQQGN VFSCSVMHEA LHNHYTQKSL SLSPGKHHHH HH