Recombinant humaan CDA-eiwit

CDA (Cytidine deaminase) is an enzyme that scavenges exogenous and endogenous cytidine and 2-deoxycytidine for uMP synthesis. This protein is one of several deaminases responsible for maintaining the cellular pyrimidine pool. The protein also catalyzes the deamination of chemotherapeutic cytosine nucleoside analogs such as Ara-C and 5-azacytidine, which results in the loss of their cytotoxic and antitumor function. It can form homotetramers and is mainly expressed in granulocytes. Recombinant human CDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 10,000 pmol/min/ug, and is defined as the amount of required to deaminate 1.0 pmole of cytidine per min at pH 7.5 at 25C.

Cytidine deaminase, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase, Cytidine deaminase

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 10,000pmol/min/ug, and is defined as the amount of required to deaminate 1.0pmole of cytidine per min at pH 7.5 at 25C.

18.3 kDa (166aa) confirmed by MALDI-TOF

CDA, Cytidine deaminase, CDD, Cytidine aminohydrolase, Cytosine nucleoside deaminase, Cytidine deaminase, ATGP0739-10 µg, ATGP0739-20 µg, ATGP0739-50 µg, ATGP0739-100 µg, ATGP0739-250 µg, ATGP0739-500 µg, ATGP0739-1 mg, ATGP0739-10, ATGP0739-20, ATGP0739-50, ATGP0739-100, ATGP0739-250, ATGP0739-500, ATGP0739-1

Evrard A., et al. (2010) Ther Drug Monit. 32 (1) :53-60.; ; Seeberg EC., et a/. (1998) Blood. 91 (11) :4127-35.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 2mM EDTA, 100 mM NaCl, 40% glycerol

Enzymes & Proteases

AAH54036.1

P32320

Human

MAQKRPACTL KPECVQQLLV CSQEAKQSAY CPYSHFPVGA ALLTQEGRIF KGCNIENACY PLGICAERTA IQKAVSEGYK DFRAIAIASD MQDDFISPCG ACRQVMREFG TNWPVYMTKP DGTYIVMTVQ ELLPSSFGPE DLQKTQ