Recombinant humaan CDC26-eiwit

CDC26, also known as ANAPC12, APC12, belongs to the CDC26 family which orchestrates progression through mitosis by decorating cell-cycle regulators with ubiquitin chains. This protein is component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. The APC/C complex acts by mediating ubiquitination and subsequent degradation of target proteins. Recombinant human CDC26 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Anaphase-promoting complex subunit CDC26, ANAPC12, APC12

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

>85% by SDS-PAGE

11.9 kDa (105aa) confirmed by MALDI-TOF

CDC26, Anaphase-promoting complex subunit CDC26, ANAPC12, APC12, ATGP1095-10 µg, ATGP1095-20 µg, ATGP1095-50 µg, ATGP1095-100 µg, ATGP1095-250 µg, ATGP1095-500 µg, ATGP1095-1 mg, ATGP1095-10, ATGP1095-20, ATGP1095-50, ATGP1095-100, ATGP1095-250, ATGP1095-500, ATGP1095-1

Jin L., et al. (2008) Cell. 133:653-665; ; Gmachl M., et al. (2000) Proc. Natl. Acad. Sci. u.S.A. 97:8973-8978

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 0.1M NaCl

Cell Cycle

NP_644815

Q8NHZ8

Human

MLRRKPTRLE LKLDDIEEFE NIRKDLETRK KQKEDVEVVG GSDGEGAIGL SSDPKSREQM INDRIGYKPQ PKPNNRSSQF GSLEF