Recombinant humaan CDK1-eiwit

Cyclin-dependent kinase 1, also known as CDK1, plays a key role in the control of the eukaryotic cell cycle by modulating the centrosome cycle as well as mitotic onset; promotes G2-M transition, and regulates G1 progress and G1-S transition via association with multiple interphase cyclins. This protein is required in higher cells for entry into S-phase and mitosis. Recombinant human CDK1 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques.

Cyclin-dependent kinase 1, CDC2, CDC28A, P34CDC2

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

35.1 kDa (305aa)

CDK1, Cyclin-dependent kinase 1, CDC2, CDC28A, P34CDC2, ATGP2877-10 µg, ATGP2877-20 µg, ATGP2877-50 µg, ATGP2877-100 µg, ATGP2877-250 µg, ATGP2877-500 µg, ATGP2877-1 mg, ATGP2877-10, ATGP2877-20, ATGP2877-50, ATGP2877-100, ATGP2877-250, ATGP2877-500, ATGP2877-1

Fourest-Lieuvin A., et al. (2006) Mol. Biol. Cell. 17:1041-1050; ; Westbrook L., et al. (2007) Cancer Res. 67:11393-11401;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) 30% glycerol, 2mM DTT, 0.1mM PMSF

Cell Cycle

NP_001777

P06493

Human