Recombinant humaan CDK2-eiwit

CDK2, also known as cyclin-depentent kinase 2, is a member of the Ser/Thr protein kinase family. It is highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2. It is a catalytic subunit of the cyclin-dependent protein kinase complex, whose activity is restricted to the G1-S phase, and is essential for cell cycle G1/S phase transition. This protein associates with and is regulated by the regulatory subunits of the complex including cyclin A or E, CDK inhibitor p21Cip1 (CDKN1A) and p27Kip1 (CDKN1B) . Its activity is also regulated by protein phosphorylation. Recombinant human CDK2 protein, fused to His-tag at C-terminus, was expressed in E. coli and purified by using conventional chromatography.

Cyclin-depentent kinase 2, p33, CDK2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

35.0 kDa (306aa) confirmed by MALDI-TOF

CDK2, Cyclin-depentent kinase 2, p33, CDK2, ATGP1180-10 µg, ATGP1180-20 µg, ATGP1180-50 µg, ATGP1180-100 µg, ATGP1180-250 µg, ATGP1180-500 µg, ATGP1180-1 mg, ATGP1180-10, ATGP1180-20, ATGP1180-50, ATGP1180-100, ATGP1180-250, ATGP1180-500, ATGP1180-1

Gyuris J., et al. (1993) Cell. 75:791-803; ; Hannon G.J., et al. (1994) Proc. Natl. Acad. Sci. u.S.A. 91:1731-1735

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Cancer

NP_001789.2

P24941

Human