Recombinant humaan CDK2AP2-eiwit

CDK2AP2, also known as DOC-1R and p14, belongs to the CDK2AP family. CDK2-associated protein is thought to negatively regulate CDK2 activity by sequestering monomeric CDK2, and targeting CDK2 for proteolysis. CDK2AP2 is a regulator for self-renewal of mouse embryonic stem cells (mESCs) under permissive conditions, and cell survival during differentiation of the mESCs into terminally differentiated cell types. CDK2AP2 may be involved in the regulation of self-renewal of stem cells during early embryogenesis. Recombinant human CDK2AP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cyclin-dependent kinase 2 associated protein 2, DOC-1R, p14

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

15.5 kDa (149aa) confirmed by MALDI-TOF

CDK2AP2, cyclin-dependent kinase 2 associated protein 2, DOC-1R, p14, ATGP1868-10 µg, ATGP1868-20 µg, ATGP1868-50 µg, ATGP1868-100 µg, ATGP1868-250 µg, ATGP1868-500 µg, ATGP1868-1 mg, ATGP1868-10, ATGP1868-20, ATGP1868-50, ATGP1868-100, ATGP1868-250, ATGP1868-500, ATGP1868-1

Deshpande AM, et al. (2012) Stem Cells Dev. Jun 25.; ; Shintani, et al. (2000) Mol. Cell. Biol. (uNITED STATES) 20 (17) : 6300-7.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Cell Cycle

NP_005842

O75956

Human

MSYKPIA PAPSSTPGSS TPGPGTPVPT GSVPSPSGSV PGAGAPFRPL FNDFGPPSMG YVQAMKPPGA QGSQSTYTDL LSVIEEMGKE IRPTYAGSKS AMERLKRGII HARALVRECL AETERNART