Recombinant humaan CDK4-eiwit

Cyclin-dependent kinase 4, also known as CDK4, belongs to the Cyclin-dependent kinases (CDK) family. CDK are the catalytic subunits of the cyclin/cdk complexes, which phosphorylate substrates on threonine/serine residues. CDK4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates CDK4 activity, whereas binding to the CDK inhibitor p16 downregulates CDK4 activity. Recombinant human CDK4 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Cyclin-dependent kinase 4, CMM3, PSK-J3

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

36.1 kDa (326aa)

CDK4, Cyclin-dependent kinase 4, CMM3, PSK-J3, ATGP1724-10 µg, ATGP1724-20 µg, ATGP1724-50 µg, ATGP1724-100 µg, ATGP1724-250 µg, ATGP1724-500 µg, ATGP1724-1 mg, ATGP1724-10, ATGP1724-20, ATGP1724-50, ATGP1724-100, ATGP1724-250, ATGP1724-500, ATGP1724-1

Serrano M., et al. (1993) Nature.366:704-707.; ; Kato J Y., et al. (1994) Mol Cell Biol. 14:2713-2721.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Cell Cycle

NP_000066

P11802

Human

MGSSHHHHHH SSGLVPRGSH MGSMATSRYE PVAEIGVGAY GTVYKARDPH SGHFVALKSV RVPNGGGGGG GLPISTVREV ALLRRLEAFE HPNVVRLMDV CATSRTDREI KVTLVFEHVD QDLRTYLDKA PPPGLPAETI KDLMRQFLRG LDFLHANCIV HRDLKPENIL VTSGGTVKLA DFGLARIYSY QMALTPVVVT LWYRAPEVLL QSTYATPVDM WSVGCIFAEM FRRKPLFCGN SEADQLGKIF DLIGLPPEDD WPRDVSLPRG AFPPRGPRPV QSVVPEMEES GAQLLLEMLT FNPHKRISAF RALQHSYLHK DEGNPE