Recombinant humaan CDK5-eiwit

CDK5, also known as cell division protein kinase 5, is a member of the cyclin-dependent kinase family. It is required for proper development of the brain and to be activated, CDK5 must associate with CDK5R1 or CDK5R2. unlike other cyclin dependent kinases, it does not also require phosphorylation on the T loop so that binding with the activator is sufficient to activate the kinase. This protein is involved in the processes of neuronal maturation and migration, phosphorylating the key intracellular adaptor of the reelin signaling chain. Recombinant human CDK5 protein, fused to His-tag at N-terminus, was expressed in E. coli.

Cyclin-dependent kinase 5, PSSALRE

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

35.8 kDa (316aa)

CDK5, Cyclin-dependent kinase 5, PSSALRE, ATGP1835-10 µg, ATGP1835-20 µg, ATGP1835-50 µg, ATGP1835-100 µg, ATGP1835-250 µg, ATGP1835-500 µg, ATGP1835-1 mg, ATGP1835-10, ATGP1835-20, ATGP1835-50, ATGP1835-100, ATGP1835-250, ATGP1835-500, ATGP1835-1

Demetrick DJ. et al. (1994) Cytogenet Cell Genet. 66:72-74.; ; Meyerson M. et al. (1992) EMBO J. 11:2909-2917.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.4M urea

Metabolism

NP_004926

Q00535

Human

MGSSHHHHHH SSGLVPRGSH MGSHMQKYEK LEKIGEGTYG TVFKAKNRET HEIVALKRVR LDDDDEGVPS SALREICLLK ELKHKNIVRL HDVLHSDKKL TLVFEFCDQD LKKYFDSCNG DLDPEIVKSF LFQLLKGLGF CHSRNVLHRD LKPQNLLINR NGELKLADFG LARAFGIPVR CYSAEVVTLW YRPPDVLFGA KLYSTSIDMW SAGCIFAELA NAGRPLFPGN DVDDQLKRIF RLLGTPTEEQ WPSMTKLPDY KPYPMYPATT SLVNVVPKLN ATGRDLLQNL LKCNPVQRIS AEEALQHPYF SDFCPP