Recombinant humaan CDKN3-eiwit

Cyclin-dependent kinase inhibitor 3, also known CDKN3, belongs to the dual specificity protein phosphatase family. It was identified as a cyclin-dependent kinase inhibitor, and has been shown to interact with, and dephosphorylate CDK2 kinase, thus prevent the activation of CDK2 kinase. Also CDKN3 is important in cell cycle regulation. Recombinant human CDKN3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Cyclin-dependent kinase inhibitor 3, CDI1, CIP2, KAP, KAP1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.9 kDa (232aa) confirmed by MALDI-TOF

CDKN3, Cyclin-dependent kinase inhibitor 3, CDI1, CIP2, KAP, KAP1, ATGP0825-10 µg, ATGP0825-20 µg, ATGP0825-50 µg, ATGP0825-100 µg, ATGP0825-250 µg, ATGP0825-500 µg, ATGP0825-1 mg, ATGP0825-10, ATGP0825-20, ATGP0825-50, ATGP0825-100, ATGP0825-250, ATGP0825-500, ATGP0825-1

Maak S., et al. (2003) Genet Sel Evol. 1:157-165.; ; Gyuris J., et al. (1993) Cell. 75:791-803.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.1M NaCl

Cell Cycle

NP_005183.2

Q16667

Human

MKPPSSIQTS EFDSSDEEPI EDEQTPIHIS WLSLSRVNCS QFLGLCALPG CKFKDVRRNV QKDTEELKSC GIQDIFVFCT RGELSKYRVP NLLDLYQQCG IITHHHPIAD GGTPDIASCC EIMEELTTCL KNYRKTLIHC YGGLGRSCLV AACLLLYLSD TISPEQAIDS LRDLRGSGAI QTIKQYNYLH EFRDKLAAHL SSRDSQSRSV SR