Recombinant humaan CDO1-eiwit

Cysteine dioxygenase (CDO1) is a mammalian non-heme iron enzyme that initiates several important metabolic pathways related to pyruvate and several sulfurate compounds including sulfate, hypotaurine and taurine. This protein catalyzes the conversion of L-cysteine to cysteine sulfinic acid (cysteine sulfinate) by incorporation of dioxygen. CDO1 is critical regulator of cellular cysteine concentrations and has an important role in maintaining the hepatic concentration of intracellular free cysteine within a proper narrow range. Recombinant CDO1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

CDO, CDO 1, CDO I, Cysteine dioxygenase type 1, Cytosolic cysteine dioxygenase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.9 kDa (207aa) confirmed by MALDI-TOF

CDO, CDO 1, CDO I, CDO1, CDO-1, CDOI, CDO-I, Cysteine dioxygenase, Cysteine dioxygenase type 1, Cysteine dioxygenase type1, Cytosolic cysteine dioxygenase, ATGP0393-10 µg, ATGP0393-20 µg, ATGP0393-50 µg, ATGP0393-100 µg, ATGP0393-250 µg, ATGP0393-500 µg, ATGP0393-1 mg, ATGP0393-010, ATGP0393-020, ATGP0393-050, ATGP0393-100, ATGP0393-250, ATGP0393-500, ATGP0393-01M

Sakakibara S., et al. (1976) Biochim Biophys Acta. 422 (2) :273-9. ; ; Ramsden DB., et al. (1997) Mol Pathol. 50 (5) :269-71.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT

Redox Proteins

AAH_24241

Q16878

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMEQ TEVLKPRTLA DLIRILHQLF AGDEVNVEEV QAIMEAYESD PTEWAMYAKF DQYRYTRNLV DQGNGKFNLM ILCWGEGHGS SIHDHTNSHC FLKMLQGNLK ETLFAWPDKK SNEMVKKSER VLRENQCAYI NDSVGLHRVE NISHTEPAVS LHLYSPPFDT CHAFDQR