Recombinant humaan CENPH-eiwit

CENPH, also known as centromere protein H, belongs to the centromere protein H family. This protein is component of the CENPA-NAC (nucleosome-associated) complex that plays a central role in assembly of kinetochore proteins. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Recombinant human CENPH protein, fused to His-tag at N-terminus, was expressed in E. coli and denatured using detergent during a conventional chromatography purification process.

Centromere protein H, CENP H, Centromere protein H, ICEN35, Interphase centromere complex protein 35, Kinetochore protein CENP H, NNF1, MIND kinetochore complex component homolog, PMF1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

15.5 kDa (133aa)

CENPH, Centromere protein H, CENP H, Centromere protein H, ICEN35, Interphase centromere complex protein 35, Kinetochore protein CENP H, NNF1, MIND kinetochore complex component homolog, PMF1, ATGP1207-10 µg, ATGP1207-20 µg, ATGP1207-50 µg, ATGP1207-100 µg, ATGP1207-250 µg, ATGP1207-500 µg, ATGP1207-1 mg, ATGP1207-10, ATGP1207-20, ATGP1207-50, ATGP1207-100, ATGP1207-250, ATGP1207-500, ATGP1207-1

Saffery R., et al. (2003) Mol Cell. 12 (2) :509-16.; ; Orthaus S., et al. (2006) Biochem Biophys Res Commun. 15; ;348 (1) :36-46.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Cancer

NP_075060

Q9H3R5

Human

MGSSHHHHHH SSGLVPRGSH MLNKLIMKSQ QESWDLEEKL LDIRKKRLQL KQASESKLLE IQTEKNKQKI DLDSMENSER IKIIRQNLQM EIKITTVIQH VFQNLILGSK VNWAEDPALK EIVLQLEKNV DMM