Recombinant humaan CERT1-eiwit

CERT is a kinase that specifically phosphorylates the N-terminal region of the non-collagenous domain of the alpha 3 chain of type IV collagen, known as the Goodpasture antigen. Goodpasture disease is the result of an autoimmune response directed at this antigen. One isoform of this protein is also involved in ceramide intracellular transport. Recombinant human CERT protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Ceramide transporter 1, Collagen type IV alpha-3-binding protein, Goodpasture antigen-binding protein, GPBP, START domain-containing protein 11, StARD11, StAR-related lipid transfer protein 11, CERT, COL4A3BP

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

32.9 kDa (290aa) confirmed by MALDI-TOF

CERT, ATGP0579-10 µg, ATGP0579-20 µg, ATGP0579-50 µg, ATGP0579-100 µg, ATGP0579-250 µg, ATGP0579-500 µg, ATGP0579-1 mg, ATGP0579-10, ATGP0579-20, ATGP0579-50, ATGP0579-100, ATGP0579-250, ATGP0579-500, ATGP0579-1

Turner N., et al. (1992) J Clin Invest 89 (2) :592-601.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Immunology

NP_112729

Q9Y5P4

Human

LH WPTSLPSGDA FSSVGTHRFV QKVEEMVQNH MTYSLQDVGG DANWQLVVEE GEMKVYRREV EENGIVLDPL KATHAVKGVT GHEVCNYFWN VDVRNDWETT IENFHVVETL ADNAIIIYQT HKRVWPASQR DVLYLSVIRK IPALTENDPE TWIVCNFSVD HDSAPLNNRC VRAKINVAMI CQTLVSPPEG NQEISRDNIL CKITYVANVN PGGWAPASVL RAVAKREYPK FLKRFTSYVQ EKTAGKPILF