Recombinant humaan CETN1-eiwit

Centrin, also known as CETN1, is an EF-hand type Ca2+-binding protein. It is localized to the centrosome of interphase cells, and redistributes to the region of the spindle poles during mitosis, reflecting the dynamic behavior of the centrosome during the cell cycle. CETN1 plays an important role in the determination of centrosome position and segregation, and in the process of microtubule severing. Recombinant human CETN1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Centrin, CEN1, CETN

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

22.1 kDa (196aa) confirmed by MALDI-TOF

CETN1, Centrin, CEN1, CETN, ATGP1544-10 µg, ATGP1544-20 µg, ATGP1544-50 µg, ATGP1544-100 µg, ATGP1544-250 µg, ATGP1544-500 µg, ATGP1544-1 mg, ATGP1544-10, ATGP1544-20, ATGP1544-50, ATGP1544-100, ATGP1544-250, ATGP1544-500, ATGP1544-1

LeDizet M., et al. (1998) Am J Physiol. 275:L1145-1156.; ; Araki M., et al. (2001) J Biol Chem. 276: 18665-18672.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol,1mM DTT

Epigenomics (Transcription & Translation)

NP_004057

Q12798

Human

MASGFK KPSAASTGQK RKVAPKPELT EDQKQEVREA FDLFDVDGSG TIDAKELKVA MRALGFEPRK EEMKKMISEV DREGTGKISF NDFLAVMTQK MSEKDTKEEI LKAFRLFDDD ETGKISFKNL KRVANELGEN LTDEELQEMI DEADRDGDGE VNEEEFLRIM KKTSLY