Recombinant humaan CGREF1-eiwit

CGREF1, also known as cell growth regulator with EF-hand domain 1, is a secreted calcium ion binding protein. This protein contains two EF-hand domains and both EF-hands are required for function. It is probably digested extracellularly by an unknown serine protease generating extremely hydrophobic bioactive peptides. It mediates cell-cell adhesion in a calcium-dependent manner. In addition, CGREF1 is able to inhibit growth in several cell lines. Recombinant human CGREF1 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Cell growth regulator with EF-hand domain 1, Cell growth regulator with EF-hand domain 1, CGR11

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

32.3 kDa (305aa) confirmed by MALDI-TOF

CGREF1, Cell growth regulator with EF-hand domain 1, Cell growth regulator with EF-hand domain 1, CGR11, ATGP2921-10 µg, ATGP2921-20 µg, ATGP2921-50 µg, ATGP2921-100 µg, ATGP2921-250 µg, ATGP2921-500 µg, ATGP2921-1 mg, ATGP2921-10, ATGP2921-20, ATGP2921-50, ATGP2921-100, ATGP2921-250, ATGP2921-500, ATGP2921-1

Dolcino M. et al. (2012) Clin Dev Immunol. 2012:198956.; ; Madden SL. D.H. et al. (1996) Cancer Res. 56:5384-5390.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4)

Cell Cycle

AAH34764

Q99674

Human

MGSSHHHHHH SSGLVPRGSH MGSAPKDGVT RPDSEVQHQL LPNPFQPGQE QLGLLQSYLK GLGRTEVQLE HLSREQVLLY LFALHDYDQS GQLDGLELLS MLTAALAPGA ANSPTTNPVI LIVDKVLETQ DLNGDGLMTP AELINFPGVA LRHVEPGEPL APSPQEPQAV GRQSLLAKSP LRQETQEAPG PREEAKGQVE ARRESLDPVQ EPGGQAEADG DVPGPRGEAE GQAEAKGDAP GPRGEAGGQA EAEGDAPGPR GEAGGQAEAR ENGEEAKELP GETLESKNTQ NDFEVHIVQV ENDEI