Recombinant humaan CHCHD3-eiwit

Coiled-coil-helix-coiled-coil-helix domain containing protein 3, also known as CHCHD3, is required for maintenance of mitochondrial crista integrity and mitochondrial function. This protein may act as a scaffolding protein that stabilizes protein complexes involved in crista architecture and protein import. It has also been shown to function as a transcription factor which binds to the BAG1 promoter and represses BAG1 transcription. Recombinant human CHCHD3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Coiled-coil-helix-coiled-coil-helix domain containing protein 3, MINOS3, PPP1R22

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 80% by SDS-PAGE

28.5 kDa (250aa) confirmed by MALDI-TOF

CHCHD3, Coiled-coil-helix-coiled-coil-helix domain containing protein 3, MINOS3, PPP1R22, ATGP2378-10 µg, ATGP2378-20 µg, ATGP2378-50 µg, ATGP2378-100 µg, ATGP2378-250 µg, ATGP2378-500 µg, ATGP2378-1 mg, ATGP2378-10, ATGP2378-20, ATGP2378-50, ATGP2378-100, ATGP2378-250, ATGP2378-500, ATGP2378-1

Liu H., et al. (2012) PLoS ONE. 7:E34832-E34832; ; Alkhaja A.K., et al. (2012) Mol. Biol. Cell. 23:247-257;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.2M NaCl, 50% glycerol, 2mM DTT

Metabolism

NP_060282

Q9NX63

Human

MGSSHHHHHH SSGLVPRGSH MGSMGGTTST RRVTFEADEN ENITVVKGIR LSENVIDRMK ESSPSGSKSQ RYSGAYGASV SDEELKRRVA EELALEQAKK ESEDQKRLKQ AKELDRERAA ANEQLTRAIL RERICSEEER AKAKHLARQL EEKDRVLKKQ DAFYKEQLAR LEERSSEFYR VTTEQYQKAA EEVEAKFKRY ESHPVCADLQ AKILQCYREN THQTLKCSAL ATQYMHCVNH AKQSMLEKGG